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KMID : 0903519810240040238
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1981 Volume.24 No. 4 p.238 ~ p.244
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Thin Layer Chromatographic Determination of α - Solanine
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Abstract
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The thin Layer chromatographic determination of α-solanine in potato tuber was investigated. The solanine was extracted with methanol-chloroform mixture (2 : 1, v/v) and precipitated as the crude glycoalkaloid by the addition of ammonium hydroxide. The solanine in the crude precipitate was separated by TLC using a solvent system of 1% NH₄OH-absolute ethanol-chloroform (1:2:2, v/v). The purity of α-solanine was confirmed by infrared spectrophotometry. The α-solanine separated by TLC was determined at the wavelength of 310㎚ after coloration with concentrated sulfuric acid-1% paraformaldehyde, with the molar absorptivity of 2,090.
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